Anticonvulsant

Anticonvulsion study

1. Chemical

2. Electrical

1. Chemical

Pentamethyltetrazole (PTZ)

It will be inducing convulsion- mice is preferred 80mg/kg intraperitonial

Assessement criteria

1. Onset of jerk moment

2. Number of myoclonic jerk

2. Straub tail

4. Clonic or tonic convulsion

5. Duration of death or recovery- This is the most important criteria for analysing the effect.

Duration/latency

1. Controlled- less

2. Test- more in intensity and number should be decreased

Electroconvulsion/ supramaximal electric shock convulsion technic

— shock instead of chemical via eye or ear

– 180mv in 0.2sec time to induce convulsion

Assessment will be done

**Stroma condition

Kindling model

– best on status epilepticus model

– daily inj ptz with 30mg/kg along with control group

– daily monitoring will be observed

– minimum day of 11

– all control group will go into death.

This model is done coninously for observation of long time period. The dose given 80mg/kg is reduced half to 40mg/kg and then observation is done. For observating at a time, the first rat is started at 0 sec, second rat at 3 sec and timely accordingly. The recording chart is made and collect the no of convulsion of the rat in which time interval. (SDM, 07/13 observation)

PHOTOS:

The criteria is fixed for the observation as 1,2,3,4,5.